Dataset Information

Characterization of the leafhopper mitogenome of Mileewa alara (Hemiptera: Cicadellidae: Mileewinae) and its phylogenetic analysis.

ABSTRACT: The mitogenome data of leafhopper species Mileewa alara was assembled and annotated in this study. The results shows that length of M. alara is 16020 bp, consist of 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and one control region. The A + T content in the mitogenome was 77.9%. Phylogenetic analysis based on 13 PCGs of four Mileewa species and other 29 Cicadellidae species, each subfamily species well separated. And M. alara clustered with M. ponta. This study also raised mitogenome of Mileewa number in GenBank to four.

SUBMITTER: He H

PROVIDER: S-EPMC8009127 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Characterization of the leafhopper mitogenome of Mileewa alara (Hemiptera: Cicadellidae: Mileewinae) and its phylogenetic analysis.

He Hongli H Yang Maofa M

Mitochondrial DNA. Part B, Resources 20210328 3

The mitogenome data of leafhopper species Mileewa alara was assembled and annotated in this study. The results shows that length of M. alara is 16020 bp, consist of 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and one control region. The A + T content in the mitogenome was 77.9%. Phylogenetic analysis based on 13 PCGs of four Mileewa species and other 29 Cicadellidae species, each subfamily species well separated. And M. alara clustered with M. pont ...[more]

PMID: 33829103

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Project description:To accumulate evidence of the phylogeny of Mileewinae and the relationships among Mileewa, Processina, and Ujna genera, we sequenced the complete mitochondrial genomes of four Mileewa spp., namely, Mileewa mira, Mileewa lamellata, Mileewa sharpa, and Mileewa amplimacula. The first complete mitogenome of the genus Processina (P. sexmaculata), established by Yang, Deitz & Li from China and comprising five species, was also sequenced in this study. Annotation showed that the five mitogenomes were 14787 -15436 bp in length, and all harbored 37 typical genes. The AT content of the five mitogenomes ranged from 78.3% to 80.2%, which was similar to that of other sequenced Mileewinae species. For protein-coding genes (PCGs), ATN was the start codon, while atp8 and nad5 genes were initiated with TTG, and a great majority of them used TAA or TAG as stop codons, whereas cox2 and nad1 ended with an incomplete codon T-. All tRNAs had a typical cloverleaf secondary structure, except for trnS1, which had a reduced dihydrouridine arm. We further used 59 Membracoidea species and two outgroups to reconstruct phylogenetic trees based on 13 PCGs under an independent partition model with Bayesian inference and Maximum-likelihood methods. Among these two trees, each of the subfamilies Cicadellinae, Typhlocybinae, and Mileewinae were recovered as a monophyletic group with high support values, suggesting that Typhlocybinae was more ancient than Mileewinae and Cicadellinae. Within the Mileewinae subfamily, all species maintained the same relationships and topologies according to both the BI and ML analyses (PP > 0.8, BS > 83) as follows: (M. sharpa + (U. puerana + ((M. ponta + (M. mira + M. lamellata)) + ((M. albovittata + (M. margheritae + M. amplimacula)) + (M. rufivena + (P. sexmaculata + M. alara)))))), and the monophyly of the genera Processina, Mileewa and Ujna were not supported. This study further enriches the Mileewinae mitogenome database and will contribute to future research on the systematics, evolution, and classification of this group.

Project description:The subfamily Mileewinae in China comprises one tribe (Mileewini), four genera (Ujna, Mileewa, Processina, Anzihelus), and 71 species, yet only 11 mitochondrial genomes have been published. This study aimed to elucidate ambiguous diagnostic traits in traditional taxonomy and examined phylogenetic relationships among genera by sequencing mitochondrial genomes from 16 species. The lengths of the mitochondrial genomes ranged from 14,532 to 15,280 bp, exhibiting an AT content of 77.2%-80.7%, indicative of AT bias. Each genome contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), and 2 ribosomal RNA genes (rRNAs). Notably, the genome of the Xizang endemic Mileewa digitata measures 15,280 bp, with an AT content of 80.7%, 10,941 bp of PCGs, and a 949 bp control region (CR) followed by a 19 bp poly-A tail. Gene arrangement among the 17 genomes mirrors that of other Mileewinae species. Analyses of nucleotide diversity and evolutionary rates indicate purifying selection on PCGs, with atp8 exhibiting the greatest variability and evolutionary rate, whereas cox1 shows the least. Genetic distance studies display minimal separation among Mileewa species, specifically Mileewa nii with Mileewa margheritae and Mileewa rufivena with Mileewa ussurica, as well as the two morphs of Mileewa digitata. Phylogenetic analyses using Bayesian Inference (BI) and Maximum Likelihood (ML) generated six trees, further questioning the monophyly of the genera Mileewa, Ujna, and Processina. The reconstructed ancestral state based on the forewing patch position suggests that the common ancestor of Chinese Mileewini species was situated exclusively on the costal margin, prompting a proposed division of the tribe's species into two categories (genera). This research enriches the understanding of phylogenetic relationships within Mileewinae and contributes to the mitochondrial genome database for this group, paving the way for future taxonomic studies in China.

Dataset Information

Characterization of the leafhopper mitogenome of Mileewa alara (Hemiptera: Cicadellidae: Mileewinae) and its phylogenetic analysis.

Publications

Characterization of the leafhopper mitogenome of <i>Mileewa alara</i> (Hemiptera: Cicadellidae: Mileewinae) and its phylogenetic analysis.

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