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Characterization and phylogenetic analysis of the mitochondrial genome of Mileewa ponta (Hemiptera: Cicadellidae: Mileewinae).

ABSTRACT: The mitogenome of one Mileewinae species Mileewa ponta was sequenced and annotated according to close Cicadellidae species in this study. Annotation shows the mitogenome of M. ponta is 15999 bp in length, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. The A + T content in the mitogenome was 79.9%. Besides, we further constructed a Bayesian Inference phylogenetic tree among M. ponta, M. albovittata, M. margheritae and other Cicadellidae species. Each subfamily species clustered together, and M. ponta clustered with M. albovittata, M. margheritae. This mitogenome will contribute to the further systematics and evolution study of subfamily Mileewinae.

SUBMITTER: He H

PROVIDER: S-EPMC7782179 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:To accumulate evidence of the phylogeny of Mileewinae and the relationships among Mileewa, Processina, and Ujna genera, we sequenced the complete mitochondrial genomes of four Mileewa spp., namely, Mileewa mira, Mileewa lamellata, Mileewa sharpa, and Mileewa amplimacula. The first complete mitogenome of the genus Processina (P. sexmaculata), established by Yang, Deitz & Li from China and comprising five species, was also sequenced in this study. Annotation showed that the five mitogenomes were 14787 -15436 bp in length, and all harbored 37 typical genes. The AT content of the five mitogenomes ranged from 78.3% to 80.2%, which was similar to that of other sequenced Mileewinae species. For protein-coding genes (PCGs), ATN was the start codon, while atp8 and nad5 genes were initiated with TTG, and a great majority of them used TAA or TAG as stop codons, whereas cox2 and nad1 ended with an incomplete codon T-. All tRNAs had a typical cloverleaf secondary structure, except for trnS1, which had a reduced dihydrouridine arm. We further used 59 Membracoidea species and two outgroups to reconstruct phylogenetic trees based on 13 PCGs under an independent partition model with Bayesian inference and Maximum-likelihood methods. Among these two trees, each of the subfamilies Cicadellinae, Typhlocybinae, and Mileewinae were recovered as a monophyletic group with high support values, suggesting that Typhlocybinae was more ancient than Mileewinae and Cicadellinae. Within the Mileewinae subfamily, all species maintained the same relationships and topologies according to both the BI and ML analyses (PP > 0.8, BS > 83) as follows: (M. sharpa + (U. puerana + ((M. ponta + (M. mira + M. lamellata)) + ((M. albovittata + (M. margheritae + M. amplimacula)) + (M. rufivena + (P. sexmaculata + M. alara)))))), and the monophyly of the genera Processina, Mileewa and Ujna were not supported. This study further enriches the Mileewinae mitogenome database and will contribute to future research on the systematics, evolution, and classification of this group.

Dataset Information

Characterization and phylogenetic analysis of the mitochondrial genome of Mileewa ponta (Hemiptera: Cicadellidae: Mileewinae).

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