Dataset Information

Chromosome-level reference genome of the European wasp spider Argiope bruennichi: a resource for studies on range expansion and evolutionary adaptation.

ABSTRACT:

Background

Argiope bruennichi, the European wasp spider, has been investigated intensively as a focal species for studies on sexual selection, chemical communication, and the dynamics of rapid range expansion at a behavioral and genetic level. However, the lack of a reference genome has limited insights into the genetic basis for these phenomena. Therefore, we assembled a high-quality chromosome-level reference genome of the European wasp spider as a tool for more in-depth future studies.

Findings

We generated, de novo, a 1.67 Gb genome assembly of A. bruennichi using 21.8× Pacific Biosciences sequencing, polished with 19.8× Illumina paired-end sequencing data, and proximity ligation (Hi-C)-based scaffolding. This resulted in an N50 scaffold size of 124 Mb and an N50 contig size of 288 kb. We found 98.4% of the genome to be contained in 13 scaffolds, fitting the expected number of chromosomes (n = 13). Analyses showed the presence of 91.1% of complete arthropod BUSCOs, indicating a high-quality assembly.

Conclusions

We present the first chromosome-level genome assembly in the order Araneae. With this genomic resource, we open the door for more precise and informative studies on evolution and adaptation not only in A. bruennichi but also in arachnids overall, shedding light on questions such as the genomic architecture of traits, whole-genome duplication, and the genomic mechanisms behind silk and venom evolution.

SUBMITTER: Sheffer MM

PROVIDER: S-EPMC7788392 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Chromosome-level reference genome of the European wasp spider Argiope bruennichi: a resource for studies on range expansion and evolutionary adaptation.

Sheffer Monica M MM Hoppe Anica A Krehenwinkel Henrik H Uhl Gabriele G Kuss Andreas W AW Jensen Lars L Jensen Corinna C Gillespie Rosemary G RG Hoff Katharina J KJ Prost Stefan S

GigaScience 20210101 1

<h4>Background</h4>Argiope bruennichi, the European wasp spider, has been investigated intensively as a focal species for studies on sexual selection, chemical communication, and the dynamics of rapid range expansion at a behavioral and genetic level. However, the lack of a reference genome has limited insights into the genetic basis for these phenomena. Therefore, we assembled a high-quality chromosome-level reference genome of the European wasp spider as a tool for more in-depth future studies ...[more]

PMID: 33410470

Similar Datasets

Project description:Background:Sexual selection theory predicts that male investment in a current female should be a function of female density and male competition. While many studies have focused on male competition, the impact of female density on male mating investment has been widely neglected. Here, we aimed to close this gap and tested effects of mate density on male mating decisions in the orb-web spider Argiope bruennichi. Males of this species mutilate their genitalia during copulation, which reduces sperm competition and limits their mating rate to a maximum of two females (bigyny). The mating rate is frequently further reduced by female aggression and cannibalization. Males can reduce the risk of cannibalism if they jump off the female in time, but will then transfer fewer sperm. An alternative solution of this trade-off is to copulate longer, commit self-sacrifice and secure higher minimal paternity. The self-sacrificial strategy may be adaptive if prospective mating chances are uncertain. In A. bruennichi, this uncertainty may arise from quick changes in population dynamics. Therefore, we expected that males would immediately respond to information about low or high mate availability and opt for self-sacrifice after a single copulation under low mate availability. If male survival depends on information about prospective mating chances, we further predicted that under high mate availability, we would find a higher rate of males that leave the first mating partner to follow a bigynous mating strategy. Method:We used naïve males and compared their mating decisions among two treatments that differed in the number of signalling females. In the high mate availability treatment, males perceived pheromone signals from four adult, virgin females, while in the low mate availability treatment only one of four females was adult and virgin and the other three were penultimate and unreceptive. Results:Males took more time to start mate searching if mate availability was low. However, a self-sacrificial strategy was not more likely under low mate availability. We found no effects of treatment on the duration of copulation, the probability to survive the first copulation or the probability of bigyny. Interestingly, survival chances depended on male size and were higher in small males. Discussion:Our results do not support the hypothesis that mate density variation affects male mating investment, although they clearly perceived mate density, which they presumably assessed by pheromone quantity. One reason for the absence of male adjustments to mating tactics could be that adaptations to survive female attacks veil adaptations that facilitate mating decisions.

Dataset Information

Chromosome-level reference genome of the European wasp spider Argiope bruennichi: a resource for studies on range expansion and evolutionary adaptation.

Background

Findings

Conclusions

Publications

Chromosome-level reference genome of the European wasp spider Argiope bruennichi: a resource for studies on range expansion and evolutionary adaptation.

Similar Datasets

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