Dataset Information
Label-free proteomic analysis of serum exosomes from paroxysmal atrial fibrillation patients.
Ontology highlight
ABSTRACT: Background
Atrial fibrillation (AF) is the most common cardiac heterogeneous rhythm disorder. It represents a major cause of mortality and morbidity, mainly related to embolic events and heart failure. Mechanisms of AF are complex and remain incompletely understood. Recent evidence suggests exosomes are membrane-coated objects released by many cell-types. Their presence in body fluids and the variable surface composition and content render them attractive as a mechanism for potential biomarkers. However, the content of serum exosomes of AF patients has not been fully delineated.Methods
In this work, the serum exosomes from AF patients and healthy donors were used to compare changes in the exosome protein content. Exosomes were isolated from serum of AF patients and healthy donors and their purity was confirmed by Western blotting assays and transmission electron microscopy (TEM). Label-free LC-MS/MS quantitative proteomic analysis was applied to analyze protein content of serum exosomes.Results
A total of 440 exosomal protein groups were identified, differentially expressed proteins were filtrated with fold change???2.0 (AF/controls protein abundance ratio???2 or???0.5) and p value less than 0.05 (p?ConclusionsThese results revealed the composition and potential function of AF serum exosomes, thus providing a new perspective on the complement system and protein folding to AF.
SUBMITTER: Ni H
PROVIDER: S-EPMC7789314 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Publications
Label-free proteomic analysis of serum exosomes from paroxysmal atrial fibrillation patients.
Clinical proteomics 20210106 1
<h4>Background</h4>Atrial fibrillation (AF) is the most common cardiac heterogeneous rhythm disorder. It represents a major cause of mortality and morbidity, mainly related to embolic events and heart failure. Mechanisms of AF are complex and remain incompletely understood. Recent evidence suggests exosomes are membrane-coated objects released by many cell-types. Their presence in body fluids and the variable surface composition and content render them attractive as a mechanism for potential bio ...[more]
