Dataset Information
Neural progenitor cell-derived nanovesicles promote hair follicle growth via miR-100.
Ontology highlight
ABSTRACT: Background
Accumulating evidence shows that mesenchymal stem cell-derived extracellular vesicles (EVs) hold great promise to promote hair growth. However, large-scale production of EVs is still a challenge. Recently, exosome-mimetic nanovesicles (NV) prepared by extruding cells have emerged as an alternative strategy for clinical-scale production. Here, ReNcell VM (ReN) cells, a neural progenitor cell line was serially extruded to produce NV.Results
ReN-NV were found to promote dermal papilla cell (DPC) proliferation. In addition, in a mouse model of depilation-induced hair regeneration, ReN-NV were injected subcutaneously, resulting in an acceleration of hair follicle (HF) cycling transition at the site. The underlying mechanism was indicated to be the activation of Wnt/?-catenin signaling pathway. Furthermore, miR-100 was revealed to be abundant in ReN-NV and significantly up-regulated in DPCs receiving ReN-NV treatment. miR-100 inhibition verified its important role in ReN-NV-induced ?-catenin signaling activation.Conclusion
These results provide an alternative agent to EVs and suggest a strategy for hair growth therapy.
SUBMITTER: Cao L
PROVIDER: S-EPMC7802142 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Publications
Neural progenitor cell-derived nanovesicles promote hair follicle growth via miR-100.
Journal of nanobiotechnology 20210111 1
<h4>Background</h4>Accumulating evidence shows that mesenchymal stem cell-derived extracellular vesicles (EVs) hold great promise to promote hair growth. However, large-scale production of EVs is still a challenge. Recently, exosome-mimetic nanovesicles (NV) prepared by extruding cells have emerged as an alternative strategy for clinical-scale production. Here, ReNcell VM (ReN) cells, a neural progenitor cell line was serially extruded to produce NV.<h4>Results</h4>ReN-NV were found to promote d ...[more]