Ultrasound in the diagnosis of acute-phase decompression sickness.
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ABSTRACT:
SUBMITTER: Yanagawa Y
PROVIDER: S-EPMC7809215 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Publications
Ultrasound in the diagnosis of acute-phase decompression sickness.
Yanagawa Youichi Y Takeuchi Ikuto I Ishiyama Junzo J
Radiology case reports 20210109 3
A 53-year-old man, who performed a 44-minute dive to a depth of 21 meters, felt severe abdominal pain with dyspnea after surfacing. An ultrasound study showed a marked snowstorm pattern in the portal vein of the liver and right ventricle, and whole body computed tomography revealed multiple gas bubbles in the right ventricle, inferior vena cava, portal, mesenteric and femoral vein. He was thus diagnosed to have decompression sickness and was therefore transported to another hospital to undergo h ...[more]
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Project description:Decompression sickness (DCS) is a potentially fatal condition usually observed after scuba diving. It involves bubble formation in blood and tissues from dissolved inert gas (usually nitrogen or helium), secondary to decreases in ambient pressure (decompression).To the best of our knowledge, no study has evaluated a DCS-induced transcriptomic signature in humans. In this study, we aim to explore the evolution of leukocyte gene expression in human subjects with DCS compared to closely matched divers after uneventful diving using a hypothesis-free RNA sequencing approach .Recruitment of DCS cases (n = 7) and controls (n = 6) was carried out using specific criteria. For both DCS cases and controls, whole blood was sampled at two time points, T1 - within 8 hours of surfacing from diving and T2 - at 40-44 hours after surfacing. Prior to sampling at T2, subjects were fasted for 10 hours. Specific exclusion criteria included a) age < 18 years b) self-reported consumption of alcohol and/or strenuous physical activity before T2 c) symptoms suggestive of delayed DCS presentation in controls at T2 d) acute life-threatening clinical complications or death within 72 hours of surfacing. All cases received emergency HBO as per United States Navy Treatment Table Six between T1 and T2.For RNA isolation, 2.5mL of whole blood was collected in a PAXgene® Blood RNA Tube (PreAnalytiX, Qiagen/BD) from DCS cases and controls at both T1 and T2. The quality of RNA was evaluated by RNA Integrity Number (RIN) determination using the RNA6000 Nano protocol on an Agilent 2100 Bioanalyzer system (Agilent, USA). The RIN values for samples undergoing transcriptome analysis ranged from 7.8 to 9.3. Depletion of alpha and beta globin mRNA was carried out using the GLOBINclear™ kit (ThermoFisher Scientific). To minimize batch effects, all samples were processed simultaneously by the same investigator. RNA samples were submitted for library generation and sequencing by the Beijing Genomics Institute (BGI-Shenzhen). Briefly, poly(A) mRNA was enriched using poly(T) oligo-attached magnetic beads, followed by fragmentation. First strand cDNA synthesis was carried out using random hexamer N6 primers and reverse transcriptase. Following adaptor ligation to cDNA fragments, PCR amplification and purification, single stranded DNA circles were generated in a final library. DNA nanoballs (DNBs) were subsequently generated by rolling circle replication, which underwent paired end sequencing (100bp) on the BGI DNBseq platform.
2021-05-23 | E-MTAB-10388 | biostudies-arrayexpress
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