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Bioelectrochemically enhanced degradation of bisphenol S: mechanistic insights from stable isotope-assisted investigations.

ABSTRACT: Electroactive microbes is the driving force for the bioelectrochemical degradation of organic pollutants, but the underlying microbial interactions between electrogenesis and pollutant degradation have not been clearly identified. Here, we combined stable isotope-assisted metabolomics (SIAM) and ¹³C-DNA stable isotope probing (DNA-SIP) to investigate bisphenol S (BPS) enhanced degradation by electroactive mixed-culture biofilms (EABs). Using SIAM, six ¹³C fully labeled transformation products were detected originating via hydrolysis, oxidation, alkylation, or aromatic ring-cleavage reactions from ¹³C-BPS, suggesting hydrolysis and oxidation as the initial and key degradation pathways for the electrochemical degradation process. The DNA-SIP results further displayed high ¹³C-DNA accumulation in the genera Bacteroides and Cetobacterium from the EABs and indicated their ability in the assimilation of BPS or its metabolites. Collectively, network analysis showed that the collaboration between electroactive microbes and BPS assimilators played pivotal roles the improvement in bioelectrochemically enhanced BPS degradation.

SUBMITTER: Hou R

PROVIDER: S-EPMC7809511 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Bioelectrochemically enhanced degradation of bisphenol S: mechanistic insights from stable isotope-assisted investigations.

Hou Rui R Gan Lin L Guan Fengyi F Wang Yi Y Li Jibing J Zhou Shungui S Yuan Yong Y

iScience 20201230 1

Electroactive microbes is the driving force for the bioelectrochemical degradation of organic pollutants, but the underlying microbial interactions between electrogenesis and pollutant degradation have not been clearly identified. Here, we combined stable isotope-assisted metabolomics (SIAM) and <sup>13</sup>C-DNA stable isotope probing (DNA-SIP) to investigate bisphenol S (BPS) enhanced degradation by electroactive mixed-culture biofilms (EABs). Using SIAM, six <sup>13</sup>C fully labeled tran ...[more]

PMID: 33490921

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Project description:Untargeted approaches and thus biological interpretation of metabolomics results are still hampered by the reliable assignment of the global metabolome as well as classification and (putative) identification of metabolites. In this work we present an liquid chromatography-mass spectrometry (LC-MS)-based stable isotope assisted approach that combines global metabolome and tracer based isotope labeling for improved characterization of (unknown) metabolites and their classification into tracer derived submetabolomes. To this end, wheat plants were cultivated in a customized growth chamber, which was kept at 400 ± 50 ppm 13CO2 to produce highly enriched uniformly 13C-labeled sample material. Additionally, native plants were grown in the greenhouse and treated with either 13C9-labeled phenylalanine (Phe) or 13C11-labeled tryptophan (Trp) to study their metabolism and biochemical pathways. After sample preparation, liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis and automated data evaluation, the results of the global metabolome- and tracer-labeling approaches were combined. A total of 1,729 plant metabolites were detected out of which 122 respective 58 metabolites account for the Phe- and Trp-derived submetabolomes. Besides m/z and retention time, also the total number of carbon atoms as well as those of the incorporated tracer moieties were obtained for the detected metabolite ions. With this information at hand characterization of unknown compounds was improved as the additional knowledge from the tracer approaches considerably reduced the number of plausible sum formulas and structures of the detected metabolites. Finally, the number of putative structure formulas was further reduced by isotope-assisted annotation tandem mass spectrometry (MS/MS) derived product ion spectra of the detected metabolites. A major innovation of this paper is the classification of the metabolites into submetabolomes which turned out to be valuable information for effective filtering of database hits based on characteristic structural subparts. This allows the generation of a final list of true plant metabolites, which can be characterized at different levels of specificity.

Dataset Information

Bioelectrochemically enhanced degradation of bisphenol S: mechanistic insights from stable isotope-assisted investigations.

Publications

Bioelectrochemically enhanced degradation of bisphenol S: mechanistic insights from stable isotope-assisted investigations.

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