Electrochemical Determination of Hydroxyurea in a Complex Biological Matrix Using MoS₂-Modified Electrodes and Chemometrics.

ABSTRACT: Hydroxyurea, an oral medication with important clinical benefits in the treatment of sickle cell anemia, can be accurately determined in plasma with a transition metal dichalcogenide-based electrochemical sensor. We used a two-dimensional molybdenum sulfide material (MoS₂) selectively electrodeposited on a polycrystalline gold electrode via tailored waveform polarization in the gold electrical double layer formation region. The electro-activity of the modified electrode depends on the electrical waveform parameters used to electro-deposit MoS₂. The concomitant oxidation of the MoS₂ material during its electrodeposition allows for the tuning of the sensor's specificity. Chemometrics, utilizing mathematical procedures such as principal component analysis and multivariable partial least square regression, were used to process the electrochemical data generated at the bare and the modified electrodes, thus allowing the hydroxyurea concentrations to be predicted in human plasma. A limit-of-detection of 22 nM and a sensitivity of 37 nA cm^-2 µM^-1 were found to be suitable for pharmaceutical and clinical applications.

SUBMITTER: Cazelles R 

PROVIDER: S-EPMC7823617 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature