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Occurrence and characteristics of Escherichia coli mcr-1-like in rabbits in Shandong, China.

ABSTRACT: Polymyxin is regarded as the last retort to fight against multidrug-resistant (MDR) Enterobacteriaceae. The emergency and spread of polymyxin-associated resistance gene mcr-1 evoked great panic of no medicine to cure the bacterial infection in society. mcr-1 is widespread in domestic and wild animals. Therefore, continuous monitoring of its prevalence and characteristics is required. In this study, we used a polymerase chain reaction (PCR)-based method to detect the mcr-1 of Escherichia coli isolated from rabbits of Tai'an, China, and determined the characteristics of mcr-1-bearing plasmids. A total of 55 non-duplicated E. coli was recovered from the swabs of rabbit faeces. Plasmid profiling, plasmid and chromosome PCR, complete genome sequencing, a conjugation experiment, lactose fermentation experiment, multilocus sequence typing and polymyxin resistance tests were performed to determine the characteristics of mcr-1-bearing plasmids. 14.6% (8/55) of the specimens were mcr-1 positive. The mcr-1-positive E. coli harboured more drug-resistant genes compared with the mcr-1-negative specimens, and results showed four sequence types. Overall, these findings suggested the possible threat of the transmission of mcr-1 from rabbits to humans, especially since the gene is located on transferable plasmids making horizontal transfer relatively easy. Since food-producing animals are necessary for our daily diet, worldwide cooperation is needed in fighting the spread of this drug resistance gene to avoid human infections with MDR pathogenic bacteria.

SUBMITTER: Wang X

PROVIDER: S-EPMC7840214 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Occurrence and characteristics of Escherichia coli mcr-1-like in rabbits in Shandong, China.

Wang Xinxing X Zhai Zhenzhen Z Zhao Xiaonan X Zhang Hongna H Jiang Hanming H Wang Xuepeng X Wang Hairong H Chang Weishan W

Veterinary medicine and science 20201003 1

Polymyxin is regarded as the last retort to fight against multidrug-resistant (MDR) Enterobacteriaceae. The emergency and spread of polymyxin-associated resistance gene mcr-1 evoked great panic of no medicine to cure the bacterial infection in society. mcr-1 is widespread in domestic and wild animals. Therefore, continuous monitoring of its prevalence and characteristics is required. In this study, we used a polymerase chain reaction (PCR)-based method to detect the mcr-1 of Escherichia coli iso ...[more]

PMID: 33012114

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Project description:The spread of colistin resistance gene mcr-1 at the animal-human interface remains largely unknown. This work aimed to investigate the molecular characteristics of two extended-spectrum-?-lactamase (ESBL)-producing Escherichia coli strains with mcr-1, i.e., strains H8 and H9, isolated from the same mink farmer. In this study, five mcr-positive E. coli strains were isolated from the mink farm. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) identified two genetically unrelated MCR-1 producers (H8 and H9) from the same farmer and two clonally related MCR-1-positive isolates (M5 and M6) from two different mink samples. Additionally, a mcr-1 variant, designated mcr-1.12, was identified in isolate M4. MIC determination revealed that all of the MCR-producing strains exhibited multiresistant phenotypes but showed susceptibility to imipenem, meropenem, amikacin, and tigecycline. Replicon typing showed that mcr-1 was associated with IncHI2 plasmids in 4 cases, while the gene was located on an IncI2 plasmid in 1 case. PacBio sequencing and plasmid analysis confirmed that the mcr-1 gene was located on an ?204-kb IncHI2 plasmid in H8 and was carried by an ?61-kb IncI2 plasmid in H9. To our knowledge, this work represents the first report of the occurrence of MCR-producing isolates from mink. Moreover, our report also describes the coexistence of two different MCR-1 producers in the same farmer. It highlights that fur farms can be reservoirs of mcr-1 genes. The identification of mcr-carrying plasmids on a fur farm is of potential public health importance, as it suggests that mcr is widespread in the animal husbandry industry.IMPORTANCE Colistin resistance is a real threat for both human and animal health. The mobile colistin resistance gene mcr has contributed to the persistence and transmission of colistin resistance at the interfaces of animals, humans, and ecosystems. Although mcr genes have usually been recovered from food animals, patients, and healthy humans, transmission of mcr genes at the animal-human interface remains largely unknown. This was the first study to isolate and characterize MCR-producing isolates from mink, as well as to report the coexistence of two different MCR-1 producers in the same farmer. The characterization and analysis of two MCR-1-producing E. coli isolates may have important implications for comprehension of the transmission dynamics of these bacteria. We emphasize the importance of improved multisectorial surveillance of colistin-resistant E. coli in this region.

Dataset Information

Occurrence and characteristics of Escherichia coli mcr-1-like in rabbits in Shandong, China.

Publications

Occurrence and characteristics of Escherichia coli mcr-1-like in rabbits in Shandong, China.

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