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Probe-Seq: Method for RNA Sequencing of Specific Cell Types from Animal Tissue.

ABSTRACT: Most organs and tissues are composed of many types of cells. To characterize cellular state, various transcription profiling approaches are currently available, including whole-tissue bulk RNA sequencing, single cell RNA sequencing (scRNA-Seq), and cell type-specific RNA sequencing. What is missing in this repertoire is a simple, versatile method for bulk transcriptional profiling of cell types for which cell type-specific genetic markers or antibodies are not readily available. We therefore developed Probe-Seq, which uses hybridization of gene-specific probes to RNA markers for isolation of specific types of cells, to enable downstream FACS isolation and bulk RNA sequencing. We show that this method can enable isolation and profiling of specific cell types from mouse retina, frozen human retina, Drosophila midgut, and developing chick retina, suggesting that it is likely useful for most organisms.

SUBMITTER: Amamoto R

PROVIDER: S-EPMC7842722 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Probe-Seq: Method for RNA Sequencing of Specific Cell Types from Animal Tissue.

Amamoto Ryoji R Garcia Mauricio D MD West Emma R ER Choi Jiho J Lapan Sylvain W SW Lane Elizabeth A EA Perrimon Norbert N Cepko Constance L CL

Bio-protocol 20200920 18

Most organs and tissues are composed of many types of cells. To characterize cellular state, various transcription profiling approaches are currently available, including whole-tissue bulk RNA sequencing, single cell RNA sequencing (scRNA-Seq), and cell type-specific RNA sequencing. What is missing in this repertoire is a simple, versatile method for bulk transcriptional profiling of cell types for which cell type-specific genetic markers or antibodies are not readily available. We therefore dev ...[more]

PMID: 33659409

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