Unknown

Dataset Information

0

Mime-seq 2.0: a method to sequence microRNAs from specific mouse cell types.


ABSTRACT: The description of precise miRNA expression patterns is crucial to understand what these small RNAs contribute to animal development and physiology. High-throughput sequencing of miRNAs from individual developmental stages has provided insight into temporal regulation but often lacks the cellular resolution to link miRNA-function to the biology of distinct cell types within complex tissues. Mime-seq 2.0 takes advantage of an engineered methyltransferase to methylate miRNAs in vivo in mice in cell types of interest. This chemical small RNA-tagging approach is followed by chemo-selective, high-throughput sequencing that enables the identification of tissue- and cell type-specific miRNA profiles in a sensitive and reproducible manner. Flow cytometry data produced as part of this study to investigate phenotypic consequences of methyltransferase expression (HENMTdc-T6B) is reposited here at BioStudies.

ORGANISM(S): Mus musculus (mouse)

SUBMITTER:  

PROVIDER: S-BSST1360 | biostudies-other |

REPOSITORIES: biostudies-other

Similar Datasets

| S-SCDT-10_1038-S44318-024-00102-8 | biostudies-other
| S-EPMC7842722 | biostudies-literature
| S-EPMC3882707 | biostudies-literature
| S-EPMC5699061 | biostudies-literature
| S-EPMC2760465 | biostudies-literature
| S-EPMC6242985 | biostudies-literature
| S-EPMC10816255 | biostudies-literature
| S-EPMC7753947 | biostudies-literature
| S-EPMC8189719 | biostudies-literature
| S-EPMC3913051 | biostudies-literature