Mime-seq 2.0: a method to sequence microRNAs from specific mouse cell types.
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ABSTRACT: The description of precise miRNA expression patterns is crucial to understand what these small RNAs contribute to animal development and physiology. High-throughput sequencing of miRNAs from individual developmental stages has provided insight into temporal regulation but often lacks the cellular resolution to link miRNA-function to the biology of distinct cell types within complex tissues. Mime-seq 2.0 takes advantage of an engineered methyltransferase to methylate miRNAs in vivo in mice in cell types of interest. This chemical small RNA-tagging approach is followed by chemo-selective, high-throughput sequencing that enables the identification of tissue- and cell type-specific miRNA profiles in a sensitive and reproducible manner. Flow cytometry data produced as part of this study to investigate phenotypic consequences of methyltransferase expression (HENMTdc-T6B) is reposited here at BioStudies.
ORGANISM(S): Mus musculus (mouse)
SUBMITTER:
PROVIDER: S-BSST1360 | biostudies-other |
REPOSITORIES: biostudies-other
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