Comparison of DNA sequencing and morphological identification techniques to characterize environmental fungal communities.
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ABSTRACT:
SUBMITTER: Shinohara N
PROVIDER: S-EPMC7846767 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Publications
Comparison of DNA sequencing and morphological identification techniques to characterize environmental fungal communities.
Shinohara Naohide N Woo Cheolwoon C Yamamoto Naomichi N Hashimoto Kazuhiro K Yoshida-Ohuchi Hiroko H Kawakami Yuji Y
Scientific reports 20210129 1
Culture-independent DNA sequencing of fungal internal transcribed spacer 2 (ITS2) region was compared to a culture-dependent morphological identification technique to characterize house dust-borne fungal communities. The abundant genera were Aspergillus, Wallemia, Cladosporium, and Penicillium. Statistically significant between-method correlations were observed for Wallemia and Cladosporium (Spearman's ρ = 0.75 and 0.72, respectively; p < 0.001). Penicillium tended to be detected with much highe ...[more]
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