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A 12-week aerobic exercise improves metabolic function with lower adipose tissue and ectopic fat in high fat diet fed rats.

ABSTRACT: Investigations of long-term exercise interventions in humans to reverse obesity is expensive and is hampered by poor compliance and confounders. In this study, we investigated intrahepatic and muscle fat, visceral and subcutaneous fat-pads, plasma metabolic profile and skeletal muscle inflammatory markers in response to 12-weeks aerobic exercise in an obese rodent model. Six week old male Wistar rats (n=20) were randomized to chow-fed control (Control, n=5), sedentary high fat diet (HFD, n=5), chow-fed exercise (Exercise, n=5) and HFD-fed exercise (HFD+Exercise, n=5) groups. The exercise groups were subjected to 12 week of motorized treadmill running at a speed of 18 m/min for 30 min/day. Differences in post-intervention measures were assessed by ANCOVA, adjusted for baseline body-weight and pre-intervention measures, where available. Post-hoc analyses were performed with Bonferroni correction. Plasma metabolic profile was worsened and fat-pads, ectopic fat in muscle and liver and inflammatory markers in skeletal muscle were elevated in sedentary HFD-fed animals relative to chow-fed controls. HFD+Exercise animals had significantly lower leptin (P=0.0004), triglycerides (P=0.007), HOMA-IR (P=0.065) intramyocellular lipids (P=0.003), intrahepatic lipids (P<0.0001), body fat % (P=0.001), subcutaneous adipose tissue (P<0.0001), visceral adipose (P<0.0001) and total fatmass (P<0.0001), relative to sedentary HFD-fed animals, despite only modestly lower body-weight. mRNA expression of inflammatory markers Interleukin 6 (IL6) and Tumor necrosis factor alpha (TNF?) were also reduced with aerobic exercise in skeletal muscle. Our results suggest that 12 week of aerobic exercise training is effective in improving metabolic health, fat depots, ectopic fat and inflammation even against a high fat dietary background.

SUBMITTER: Gopalan V

PROVIDER: S-EPMC7846962 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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A 12-week aerobic exercise intervention results in improved metabolic function and lower adipose tissue and ectopic fat in high-fat diet fed rats.

Gopalan Venkatesh V Yaligar Jadegoud J Michael Navin N Kaur Kavita K Anantharaj Rengaraj R Verma Sanjay Kumar SK Sadananthan Suresh Anand SA Le Giang Thi Thu GTT Goh Jorming J Velan S Sendhil SS

Bioscience reports 20210101 1

Investigations of long-term exercise interventions in humans to reverse obesity is expensive and is hampered by poor compliance and confounders. In the present study, we investigated intrahepatic and muscle fat, visceral and subcutaneous fat pads, plasma metabolic profile and skeletal muscle inflammatory markers in response to 12-week aerobic exercise in an obese rodent model. Six-week-old male Wistar rats (n=20) were randomized to chow-fed control (Control, n=5), sedentary high-fat diet (HFD, n ...[more]

PMID: 33432988

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Project description:BackgroundIndividuals with celiac disease must follow a strict gluten-free diet (GFD) in order to avoid negative short- and long-term health consequences. Unfortunately, many people with celiac disease report poor quality of life (QoL) despite following a strict GFD, and up to 30% still report negative symptoms (eg, gastrointestinal upset).PurposeThe purpose of the MOVE-C (understanding the relationship between the MicrobiOme, Vitality, and Exercise in Celiac disease) pilot study is to explore the effects of a 12-week supervised progressive high-intensity interval training (HIIT) and lifestyle intervention on physiological, behavioral, and psychosocial outcomes among inactive adults with celiac disease.Methods/Design: Sixty inactive adults diagnosed with celiac disease will be randomized to HIIT+ or waitlist control (WLC). Participants in the HIIT+ will engage in a 12-week HIIT + lifestyle education program. HIIT sessions will be comprised of 2 workouts per week, working up to 14 × 30-second intervals at 90% maximal heart rate (HRmax) followed by 2 minutes recovery at 50% HRmax. The 6 biweekly lifestyle sessions will involve education on the promotion of a whole foods GFD, sleep hygiene, psychosocial coping skills (eg, self-compassion), and self-regulatory skills to master changes in behaviors. Assessments will occur at pre and post 12-week intervention and 3-month follow-up. WLC participants will be offered a 12-week HIIT program + online lifestyle education sessions after completing the final assessment. The primary outcomes are QoL and gut microbiota composition assessed with 16S rRNA sequencing. The secondary outcomes are markers of metabolic syndrome (waist circumference, fasting glucose, serum lipids, blood pressure, and body composition), gastrointestinal symptoms, sleep quality, adherence to a GFD, exercise behavior, self-regulatory efficacy, and self-compassion. It is hypothesized that participants in the HIIT+ will experience improvements in all outcomes when compared to those in the WLC. These improvements are expected to be maintained at the 3-month follow-up.DiscussionThe findings from this study will advance the knowledge regarding the effects of HIIT and lifestyle education on key outcomes for an at-risk chronic disease population. Furthermore, the findings can be used to inform future programs to improve fitness and physical and mental health outcomes for people with celiac disease.

Dataset Information

A 12-week aerobic exercise improves metabolic function with lower adipose tissue and ectopic fat in high fat diet fed rats.

Publications

A 12-week aerobic exercise intervention results in improved metabolic function and lower adipose tissue and ectopic fat in high-fat diet fed rats.

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