Diagnostic potential of three serum microRNAs as biomarkers for equine sarcoid disease in horses and donkeys.

ABSTRACT:

Background

MicroRNAs (miRNAs) are potential biomarkers for equine sarcoids (ES).

Objectives

To assess eca-miR-331, eca-miR-100, and eca-miR-1 as serum biomarkers for ES disease.

Animals

Sixty-eight ES cases (56 horses, 12 donkeys), 69 tumor-free controls (60 horses, 9 donkeys), and 20 horses with other skin tumors.

Methods

For this case-control study, expression of serum eca-miR-331, eca-miR-100, and eca-miR-1 in ES-affected equids was compared to tumor-free age-, sex-, and breed-matched control horses and donkeys with other skin tumors using reverse transcription quantitative PCR (polymerase chain reaction) for relative miRNA quantification. Biological, preanalytical, and clinical variable influences on miRNA expression were examined. Receiver operator characteristic (ROC) curve analyses were used to determine differences in miRNA expression between groups.

Results

The expression of eca-miR-100 was affected by age (P = .003) and expression of eca-miR-100 and eca-miR-1 were affected by hemolysis (both P?Conclusions and clinical importanceSerum eca-miR-331 expression is neither sensitive nor specific enough as a single ES biomarker. If combined with other miRNAs, it may be helpful for ES diagnosis.

SUBMITTER: Unger L 

PROVIDER: S-EPMC7848377 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature