Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction.

ABSTRACT: Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles.

SUBMITTER: Niemoller C 

PROVIDER: S-EPMC7851132 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature