Dataset Information

Localization and Composition of Fructans in Stem and Rhizome of Agave tequilana Weber var. azul.

ABSTRACT: Methodology combining mass spectrometry imaging (MSI) with ion mobility separation (IMS) has emerged as a biological imaging technique due to its versatility, sensitivity and label-free approach. This technique has been shown to separate isomeric compounds such as lipids, amino acids, carboxylic acids and carbohydrates. This report describes mass spectrometry imaging in combination with traveling-wave ion mobility separation and matrix-assisted laser desorption/ionization (MALDI). Positive ionization mode was used to locate fructans on tissue printed sections of Agave rhizome and stem tissue and distinguished fructan isoforms. Here we show the location of fructans ranging from DP3 to DP17 to be differentially abundant across the stem tissue and for the first time, experimental collision cross sections of endogenous fructan structures have been collected, revealing at least two isoforms for fructans of DP4, DP5, DP6, DP7, DP8, DP10, and DP11. This demonstrates that complex fructans such as agavins can be located and their isoforms resolved using a combination of MALDI, IMS, and MSI, without the need for extraction or derivatization. Use of this methodology uncovered patterns of fructan localization consistent with functional differences where higher DP fructans are found toward the central section of the stem supporting a role in long term carbohydrate storage whereas lower DP fructans are concentrated in the highly vascularized central core of rhizomes supporting a role in mobilization of carbohydrates from the mother plant to developing offsets. Tissue specific patterns of expression of genes encoding enzymes involved in fructan metabolism are consistent with fructan structures and localization.

SUBMITTER: Perez-Lopez AV

PROVIDER: S-EPMC7855178 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Localization and Composition of Fructans in Stem and Rhizome of <i>Agave tequilana</i> Weber var. azul.

Pérez-López Arely V AV Simpson June J Clench Malcolm R MR Gomez-Vargas Alan D AD Ordaz-Ortiz José J JJ

Frontiers in plant science 20210120

Methodology combining mass spectrometry imaging (MSI) with ion mobility separation (IMS) has emerged as a biological imaging technique due to its versatility, sensitivity and label-free approach. This technique has been shown to separate isomeric compounds such as lipids, amino acids, carboxylic acids and carbohydrates. This report describes mass spectrometry imaging in combination with traveling-wave ion mobility separation and matrix-assisted laser desorption/ionization (MALDI). Positive ioniz ...[more]

PMID: 33552101

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Project description:Abstract Agave (Agave tequilana var. azul) is considered a crop with low genetic diversity because it has been propagated vegetatively for centuries for commercial purposes, and consequently, it could be equally susceptible to pests and diseases. However, the present study employs plant material derived from field-grown plants exhibiting phenotypic variability in susceptibility to agave wilt. The offshoots from rhizomes of these plants were reproduced in vitro and classified as potentially resistant or susceptible. Amplified fragment length polymorphism analysis confirmed wide genetic differences among individuals, but these differences were not correlated with the observed phenotypic variability in resistance. Propagated plantlets were inoculated with Fusarium solani in two time-lapse confrontations for 72 h and 30 days. The early biochemical response showed statistically superior levels in the accumulation of shikimic acid, phenolic compounds, and chitinase activity in potentially resistant plantlets. There was an inverse correlation of these early biochemical responses and salicylic acid and the incidence of diseased root cells in isogenic plantlets in the 30-day confrontation with F. solani, suggesting that these activities and accumulation of molecules were primordial in the defence against this pathogen. Agave tequilanavar. azul has been propagated vegetatively for centuries for commercial purposes, and consequently, is believed to be uniformly susceptible to diseases. However, the present study employs plants exhibiting apparent variability in susceptibility to agave wilt as well as genetic diversity. These plants were inoculated with the pathogen Fusarium solaniand the early biochemical response showed differences in the accumulation of defence-associated molecules and in the level of root damage after 30 days of confrontation, suggesting that in the population there exist individuals with the potential to be selected for their resistance to economically important diseases of this crop.

Dataset Information

Localization and Composition of Fructans in Stem and Rhizome of Agave tequilana Weber var. azul.

Publications

Localization and Composition of Fructans in Stem and Rhizome of <i>Agave tequilana</i> Weber var. azul.

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