Browse
Submit Data
Databases
API
Help

Dataset Information

31 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Polyphosphazenes enable durable, hemocompatible, highly efficient antibacterial coatings.

ABSTRACT: Biocompatible antibacterial coatings are highly desirable to prevent bacterial colonization on a wide range of medical devices from hip implants to skin grafts. Traditional polyelectrolytes are unable to directly form coatings with cationic antibiotics at neutral pH and suffer from high degrees of antibiotic release upon exposure to physiological concentrations of salt. Here, novel inorganic-organic hybrid polymer coatings based on direct layer-by-layer assembly of anionic polyphosphazenes (PPzs) of various degrees of fluorination with cationic antibiotics (polymyxin B, colistin, gentamicin, and neomycin) are reported. The coatings displayed low levels of antibiotic release upon exposure to salt and pH-triggered response of controlled doses of antibiotics. Importantly, coatings remained highly surface active against Escherichia coli and Staphylococcus aureus, even after 30 days of pre-exposure to physiological conditions (bacteria-free) or after repeated bacterial challenge. Moreover, coatings displayed low (<1%) hemolytic activity for both rabbit and porcine blood. Coatings deposited on either hard (Si wafers) or soft (electrospun fiber matrices) materials were non-toxic towards fibroblasts (NIH/3T3) and displayed controllable fibroblast adhesion via PPz fluorination degree. Finally, coatings showed excellent antibacterial activity in ex vivo pig skin studies. Taken together, these results suggest a new avenue to form highly tunable, biocompatible polymer coatings for medical device surfaces.

SUBMITTER: Albright V

PROVIDER: S-EPMC7855754 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

New design for highly durable infrared-reflective coatings.

Project description:The fundamental challenge in designing durable infrared-reflective coatings is achieving the ideal combination of both high reflectivity and durability. Satisfying these competing demands is traditionally achieved by deposition of durable layers on highly reflective metals. We overturn the traditional logic of 'first reflectivity and then durability' and propose an alternative of 'first durability and then reflectivity': First, a transition-metal compound is selected as a durable base; then its reflectivity is improved by incorporating silver/gold to form an alloy or by overcoating a multilayer stack. Two validation experiments prove that the new strategy works extremely well: the coatings thus obtained have infrared reflectivities close to that of aluminum, and their hardness and acid and salt corrosion resistances are 27-50, 400-1 500 and 7 500-25 000 times that of aluminum. The traditional mirror coating (e.g., Al/SiO2 films) is more suitable for moderate environments, while our mirror coating that was obtained by the new strategy (e.g., an Ag-doped hafnium nitride film) is more suitable for harsh environments, such as ones with dust, windblown sand, moisture, acid rain or salt fog. This work opens up new opportunities for highly durable infrared-reflective coatings and rejuvenates the study of transition metal compounds in a completely new area of optics.

| S-EPMC6060051 | biostudies-literature

Small molecules enable highly efficient neuronal conversion of human fibroblasts

Project description:Forced expression of pro-neural transcription factors was shown to mediate direct neuronal conversion of human fibroblasts. Since neurons are postmitotic, the conversion efficiency represents an important parameter. Here we present a minimalist approach combining two factor neuronal programming with small molecule-based inhibition of GSK3ß and SMAD signaling, which gives rise to functional neuron-like cells (iNs) of various neurotransmitter phenotypes with an overall yield of up to >200% and a final neuronal purity of up to >80%. Timcourse of reprogramming of fibroblasts towards an neuronal phenotype in two independent fibroblast lines

2012-04-07 | E-GEOD-36246 | biostudies-arrayexpress

Highly efficient and durable antibacterial cotton fabrics finished with zwitterionic polysulfobetaine by one-step eco-friendly strategy.

Project description:In this work, a novel formulation of polysulfobetaine, poly (sulfobetaine-acrylamide-allyl glycidyl ether) (PSPB-AM-AGE), was synthesized and grafted onto cotton. The synthesis of PSPB-AM-AGE and its grafting on the cotton fabrics were confirmed by FTIR, XPS and SEM. The PSPB-AM-AGE treated cotton fabrics exhibited a high level of antibacterial rate against both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), which are 95.18% and 98.74%, separately, as well as a good laundry durability. The mechanical tests showed that the essential cotton properties can be largely preserved in the treatment process. Moreover, the hydrophilicity, air and water permeability of the cotton were improved after treated with PSPB-AM-AGE, indicating a better wearing comfort performance. The whiteness of the cotton fabrics did not decrease significantly. The safety evaluation demonstrated that PSPB-AM-AGE had no cytotoxicity. The developed antibacterial finishing introduced a new method to apply polysulfobetaine interfaced on cellulose, providing great potential for biomedical fabric application.

| S-EPMC7653989 | biostudies-literature

Highly efficient and durable antimicrobial nanocomposite textiles.

Project description:Healthcare associated infections cause millions of hospitalizations and cost billions of dollars every year. A potential solution to address this problem is to develop antimicrobial textile for healthcare fabrics (hospital bedding, gowns, lab coats, etc.). Metal nanoparticle-coated textile has been proven to possess antimicrobial properties but have not been adopted by healthcare facilities due to risks of leaching and subsequent loss of function, toxicity, and environmental pollution. This work presents the development and testing of antimicrobial zinc nanocomposite textiles, fabricated using a novel Crescoating process. In this process, zinc nanoparticles are grown in situ within the bulk of different natural and synthetic fabrics to form safe and durable nanocomposites. The zinc nanocomposite textiles show unprecedented microbial reduction of 99.99% (4 log10) to 99.9999% (6 log10) within 24 h on the most common Gram-positive and Gram-negative bacteria, and fungal pathogens. Furthermore, the antimicrobial activity remains intact even after 100 laundry cycles, demonstrating the high longevity and durability of the textile. Independent dermatological evaluation confirmed that the novel textile is non-irritating and hypoallergenic.

| S-EPMC9568944 | biostudies-literature

Small molecules enable highly efficient neuronal conversion of human fibroblasts

2012-04-08 | GSE36246 | GEO

Triboelectric Yarns with Electrospun Functional Polymer Coatings for Highly Durable and Washable Smart Textile Applications.

Project description:Triboelectric generators are excellent candidates for smart textiles applications due to their ability to convert mechanical energy into electrical energy. Such devices can be manufactured into yarns by coating a conductive core with a triboelectric material, but current triboelectric yarns lack the durability and washing resistance required for textile-based applications. In this work, we develop a unique triboelectric yarn comprising a conducting carbon nanotube (CNT) yarn electrode coated with poly(vinylidene fluoride) (PVDF) fibers deposited by a customized electrospinning process. We show that the electrospun PVDF fibers adhere extremely well to the CNT core, producing a uniform and stable triboelectric coating. The PVDF-CNT coaxial yarn exhibits remarkable triboelectric energy harvesting during fatigue testing with a 33% power output improvement and a peak power density of 20.7 μW cm-2 after 200 000 fatigue cycles. This is potentially due to an increase in the active surface area of the PVDF fiber coating upon repeated contact. Furthermore, our triboelectric yarn meets standard textile industry benchmarks for both abrasion and washing by retaining functionality over 1200 rubbing cycles and 10 washing cycles. We demonstrate the energy harvesting and motion sensing capabilities of our triboelectric yarn in prototype textile-based applications, thereby highlighting its applicability to smart textiles.

| S-EPMC8045025 | biostudies-literature

Highly Efficient Antimicrobial and Antifouling Surface Coatings with Triclosan-Loaded Nanogels.

Project description:Multifunctional nanogel coatings provide a promising antimicrobial strategy against biomedical implant-associated infections. Nanogels can create a hydrated surface layer to promote antifouling properties effectively. Further modification of nanogels with quaternary ammonium compounds (QACs) potentiates antimicrobial activity owing to their positive charges along with the presence of a membrane-intercalating alkyl chain. This study effectively demonstrates that poly(N-isopropylacrylamide-co-N-[3(dimethylamino)propyl]methacrylamide) (P(NIPAM-co-DMAPMA)-based nanogel coatings possess antifouling behavior against S. aureus ATCC 12600, a Gram-positive bacterium. Through the tertiary amine in the DMAPMA comonomer, nanogels are quaternized with a 1-bromo-dodecane chain via an N-alkylation reaction. The alkylation introduces the antibacterial activity due to the bacterial membrane binding and the intercalating ability of the aliphatic QAC. Subsequently, the quaternized nanogels enable the formation of intraparticle hydrophobic domains because of intraparticle hydrophobic interactions of the aliphatic chains allowing for Triclosan incorporation. The coating with Triclosan-loaded nanogels shows a killing efficacy of up to 99.99% of adhering bacteria on the surface compared to nonquaternized nanogel coatings while still possessing an antifouling activity. This powerful multifunctional coating for combating biomaterial-associated infection is envisioned to greatly impact the design approaches for future clinically applied coatings.

| S-EPMC7775744 | biostudies-literature

Long-lasting renewable antibacterial porous polymeric coatings enable titanium biomaterials to prevent and treat peri-implant infection.

Project description:Peri-implant infection is one of the biggest threats to the success of dental implant. Existing coatings on titanium surfaces exhibit rapid decrease in antibacterial efficacy, which is difficult to promisingly prevent peri-implant infection. Herein, we report an N-halamine polymeric coating on titanium surface that simultaneously has long-lasting renewable antibacterial efficacy with good stability and biocompatibility. Our coating is powerfully biocidal against both main pathogenic bacteria of peri-implant infection and complex bacteria from peri-implantitis patients. More importantly, its antibacterial efficacy can persist for a long term (e.g., 12~16 weeks) in vitro, in animal model, and even in human oral cavity, which generally covers the whole formation process of osseointegrated interface. Furthermore, after consumption, it can regain its antibacterial ability by facile rechlorination, highlighting a valuable concept of renewable antibacterial coating in dental implant. These findings indicate an appealing application prospect for prevention and treatment of peri-implant infection.

| S-EPMC8175680 | biostudies-literature

PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens.

Project description:Sensitive and reproducible diagnostics are fundamental to containing the spread of existing and emerging pathogens. Despite the reliance of clinical virology on qPCR, technical challenges persist that compromise their reliability for sustainable epidemic containment as sequence instability in probe-binding regions produces false-negative results. We systematically violated canonical qPCR design principles to develop a Pan-Degenerate Amplification and Adaptation (PANDAA), a point mutation assay that mitigates the impact of sequence variation on probe-based qPCR performance. Using HIV-1 as a model system, we optimized and validated PANDAA to detect HIV drug resistance mutations (DRMs). Ultra-degenerate primers with 3' termini overlapping the probe-binding site adapt the target through site-directed mutagenesis during qPCR to replace DRM-proximal sequence variation. PANDAA-quantified DRMs present at frequency ≥5% (2 h from nucleic acid to result) with a sensitivity and specificity of 96.9% and 97.5%, respectively. PANDAA is an innovative advancement with applicability to any pathogen where target-proximal genetic variability hinders diagnostic development.

| S-EPMC7892852 | biostudies-literature

UV-durable self-cleaning coatings for autonomous driving.

Project description:A key technology to ensure the safety and accuracy of autonomous driving for future transportation is the cleanliness of the sensor surfaces for accurate signal reading. This study focuses on hydrophobic coatings with self-cleaning performances and UV durability, their possible degradation mechanism of static water contact angle (sWCA), and the effect of the hydrophobic surface on camera image quality. The UV-durable hydrophobic coatings are applied by a spray process followed by a thermal curing. The UV-durable hydrophobic coatings are evaluated on a vision camera under lab-simulated weathering conditions such as rain, mud, fog, and bugs, on samples as-prepared and after various hours of Weather-Ometer® weathering. The results indicate that the sWCA degradation of the UV-durable hydrophobic coatings during accelerated weathering is corresponding to the loss of fluorine (F) atomic percentage in the coatings, and the vision camera imaging quality improves significantly with the UV-durable hydrophobic coatings in comparison to an uncoated surface. The self-cleaning performances of the UV-durable hydrophobic coatings, as measured by two metrics using signal-to-noise ratio and modulation transfer function 50 loss (MTF50loss), linearly correlate with sWCA of the coatings. The UV-durable hydrophobic coatings on the sensor surface will significantly benefit autonomous driving specifically for accurate signal reading under inclement weather.

| S-EPMC10997611 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data