Ontology highlight
ABSTRACT:
SUBMITTER: Antonescu ON
PROVIDER: S-EPMC7857580 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Antonescu Oana N ON Rasmussen Andreas A Damm Nicole A M NAM Heidemann Ditte F DF Popov Roman R Nesterov-Mueller Alexander A Johansson Kristoffer E KE Winther Jakob R JR
PloS one 20210203 2
Split fluorescent proteins have wide applicability as biosensors for protein-protein interactions, genetically encoded tags for protein detection and localization, as well as fusion partners in super-resolution microscopy. We have here established and validated a novel platform for functional analysis of leave-one-out split fluorescent proteins (LOO-FPs) in high throughput and with rapid turnover. We have screened more than 12,000 variants of the beta-strand split fragment using high-density pep ...[more]