Development of improved method to identify and analyze lung fibrocytes with flow cytometry in a reporter mouse strain.

ABSTRACT:

Introduction

Fibrocytes are emerging myeloid-derived circulating cells that can migrate into damaged tissues and usually contribute to their repair. Key features of fibrocytes include the expression myeloid markers, production of extracellular matrix proteins, and secretion of various humoral factors that activate resident fibroblasts; they also have the potential to differentiate into fibroblasts. However, no specific surface markers have been identified to identify fibrocytes in vivo. One reason could be that the method used to detect fibrocytes requires intracellular collagen staining.

Methods

In the present study, to establish an improved method for the detection of lung fibrocytes and to analyze viable fibrocytes, we used collagen I(α)2-green fluorescent protein (Col-GF

SUBMITTER: Kawano H 

PROVIDER: S-EPMC7860606 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature