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Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells.


ABSTRACT: The rice cell suspension culture system is a good way to produce recombinant human proteins, owing to its high biosafety and low production cost. Human Octamer-binding Transcription Factor 4 (Oct4) is a fundamental transcription factor responsible for maintaining human pluripotent embryonic stem cells. Recombinant Oct4 protein has been used to induce pluripotent stem cells. In this study, recombinant Oct4 proteins are produced via a sugar starvation-inducible ?Amy3/RAmy3D promoter-signal peptide-based rice recombinant protein expression system. Oct4 mRNAs accumulate in the transgenic rice suspension cells under sugar starvation. The Oct4 recombinant protein is detected in the transgenic rice suspension cells, and its highest yield is approximately 0.41% of total cellular soluble proteins after one day of sugar starvation. The rice cell-synthesized recombinant human Oct4 protein show DNA-binding activity in vitro, which implies that the protein structure is correct for enabling specific binding to the target DNA motif.

SUBMITTER: Huang LF 

PROVIDER: S-EPMC7866794 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells.

Huang Li-Fen LF   Sinaga Desyanti Saulina DS   Tan Chia-Chun CC   Hsieh Shu-Ju Micky SM   Huang Chi-Hung CH  

International journal of molecular sciences 20210130 3


The rice cell suspension culture system is a good way to produce recombinant human proteins, owing to its high biosafety and low production cost. Human Octamer-binding Transcription Factor 4 (Oct4) is a fundamental transcription factor responsible for maintaining human pluripotent embryonic stem cells. Recombinant Oct4 protein has been used to induce pluripotent stem cells. In this study, recombinant Oct4 proteins are produced via a sugar starvation-inducible <i>αAmy3</i>/<i>RAmy3D</i> promoter-  ...[more]

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