In vivo imaging of a PVD neuron in Caenorhabditis elegans.
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ABSTRACT:
SUBMITTER: Wang X
PROVIDER: S-EPMC7868634 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Publications
<i>In vivo</i> imaging of a PVD neuron in <i>Caenorhabditis elegans</i>.
Wang Xinjian X Li Tingting T Hu Jiawen J Feng Zhigang Z Zhong Rui R Nie Wang W Yang Xiaoyan X Zou Yan Y
STAR protocols 20210204 1
The nematode <i>Caenorhabditis elegans</i> nociceptive PVD neurons have highly ordered dendritic branches, making this an ideal model to study the development and organization of dendrites. A <i>ser-2</i>-promoter-driven GFP reporter line <i>wyIs592</i>[<i>ser-2prom-3p::myr-</i>GFP] provides a comprehensive visualization of PVD anatomy. Here, we describe the detailed procedures for imaging a PVD neuron using <i>wyIs592</i> at late L4 larval stage <i>in vivo</i> by confocal microscopy. This proto ...[more]
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