Ontology highlight
ABSTRACT:
SUBMITTER: Munson MJ
PROVIDER: S-EPMC7887203 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Munson Michael J MJ O'Driscoll Gwen G Silva Andreia M AM Lázaro-Ibáñez Elisa E Gallud Audrey A Wilson John T JT Collén Anna A Esbjörner Elin K EK Sabirsh Alan A
Communications biology 20210216 1
RNA-based therapies have great potential to treat many undruggable human diseases. However, their efficacy, in particular for mRNA, remains hampered by poor cellular delivery and limited endosomal escape. Development and optimisation of delivery vectors, such as lipid nanoparticles (LNPs), are impeded by limited screening methods to probe the intracellular processing of LNPs in sufficient detail. We have developed a high-throughput imaging-based endosomal escape assay utilising a Galectin-9 repo ...[more]