Ontology highlight
ABSTRACT:
SUBMITTER: Kato S
PROVIDER: S-EPMC7894531 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Kato Shunsuke S Onoda Akira A Taniguchi Naomasa N Schwaneberg Ulrich U Hayashi Takashi T
Chembiochem : a European journal of chemical biology 20201109 4
Directed evolution of Cp*Rh<sup>III</sup> -linked nitrobindin (NB), a biohybrid catalyst, was performed based on an in vitro screening approach. A key aspect of this effort was the establishment of a high-throughput screening (HTS) platform that involves an affinity purification step employing a starch-agarose resin for a maltose binding protein (MBP) tag. The HTS platform enables efficient preparation of the purified MBP-tagged biohybrid catalysts in a 96-well format and eliminates background i ...[more]