Ontology highlight
ABSTRACT:
SUBMITTER: Joyce P
PROVIDER: S-EPMC7894553 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20201123 4
There is an urgent demand for analytic approaches that enable precise and representative quantification of the transport of biologically active compounds across cellular membranes. In this study, we established a new means to monitor membrane permeation kinetics, using total internal reflection fluorescence microscopy confined to a ≈500 nm thick mesoporous silica substrate, positioned underneath a planar supported cell membrane mimic. This way, we demonstrate spatiotemporally resolved membrane p ...[more]