Ontology highlight
ABSTRACT:
SUBMITTER: Lee H
PROVIDER: S-EPMC7895935 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Lee Hyunji H Lee Seonghyun S Baek Gayoung G Kim Annie A Kang Beum-Chang BC Seo Huiyun H Kim Jin-Soo JS
Nature communications 20210219 1
DddA-derived cytosine base editors (DdCBEs), composed of the split interbacterial toxin DddA<sub>tox</sub>, transcription activator-like effector (TALE), and uracil glycosylase inhibitor (UGI), enable targeted C-to-T base conversions in mitochondrial DNA (mtDNA). Here, we demonstrate highly efficient mtDNA editing in mouse embryos using custom-designed DdCBEs. We target the mitochondrial gene, MT-ND5 (ND5), which encodes a subunit of NADH dehydrogenase that catalyzes NADH dehydration and electro ...[more]