Ontology highlight
ABSTRACT:
SUBMITTER: Wang L
PROVIDER: S-EPMC7902551 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
STAR protocols 20210216 1
Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are multi-component, ATP-driven proton pumps, which play important roles in many physiological processes by acidifying intracellular vesicles, organelles, and the extracellular milieu. Long-standing challenges in purifying mammalian V-ATPases have limited the biochemical and structural study of mammalian V-ATPase. Here, we provide a protocol for purifying milligrams of human V-ATPase and detail procedures for the reconstruction of ...[more]