Dataset Information

Functional Analysis of the Gibberellin 2-oxidase Gene Family in Peach.

ABSTRACT: Peach (Prunus persica L. Batsch) trees grow vigorously and are subject to intense pruning during orchard cultivation. Reducing the levels of endogenous gibberellins (GAs) represents an effective method for controlling branch growth. Gibberellin 2-oxidases (GA2oxs) deactivate bioactive GAs, but little is known about the GA2ox gene family in peach. In this study, we identified seven PpGA2ox genes in the peach genome, which were clustered into three subgroups: C₁₉-GA2ox-I, C₁₉-GA2ox-II, and C₂₀-GA2ox-I. Overexpressing representative genes from the three subgroups, PpGA2ox-1, PpGA2ox-5, and PpGA2ox-2, in tobacco resulted in dwarf plants with shorter stems and smaller leaves than the wild type. An analysis of the GA metabolic profiles of the transgenic plants showed that PpGA2ox-5 (a member of subgroup C₁₉-GA2ox-II) is simultaneously active against both C₁₉-GAs and C₂₀-GAs,which implied that C₁₉-GA2ox-II enzymes represent intermediates of C₁₉-GA2oxs and C₂₀-GA2oxs. Exogenous GA₃ treatment of shoot tips activated the expression of all seven PpGA2ox genes, with different response times: the C ₁₉-GA2ox genes were transcriptionally activated more rapidly than the C₂₀-GA2ox genes. GA metabolic profile analysis suggested that C₂₀-GA2ox depletes GA levels more broadly than C₁₉-GA2ox. These results suggest that the PpGA2ox gene family is responsible for fine-tuning endogenous GA levels in peach. Our findings provide a theoretical basis for appropriately controlling the vigorous growth of peach trees.

SUBMITTER: Cheng J

PROVIDER: S-EPMC7928363 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Functional Analysis of the Gibberellin 2-oxidase Gene Family in Peach.

Cheng Jun J Ma Jingjing J Zheng Xianbo X Lv Honglin H Zhang Mengmeng M Tan Bin B Ye Xia X Wang Wei W Zhang Langlang L Li Zhiqian Z Li Jidong J Feng Jiancan J

Frontiers in plant science 20210217

Peach (Prunus persica L. Batsch) trees grow vigorously and are subject to intense pruning during orchard cultivation. Reducing the levels of endogenous gibberellins (GAs) represents an effective method for controlling branch growth. Gibberellin 2-oxidases (GA2oxs) deactivate bioactive GAs, but little is known about the GA2ox gene family in peach. In this study, we identified seven PpGA2ox genes in the peach genome, which were clustered into three subgroups: C19-GA2ox-I, ...[more]

PMID: 33679834

Similar Datasets

Project description:The plant hormones gibberellins (GAs) regulate plant growth and development and are closely related to the yield of cash crops. The GA oxidases (GAoxs), including the GA2ox, GA3ox, and GA20ox subfamilies, play pivotal roles in GAs' biosynthesis and metabolism, but their classification and evolutionary pattern in Gramineae crops remain unclear. We thus conducted a comparative genomic study of GAox genes in six Gramineae representative crops, namely, Setaria italica (Si), Zea mays (Zm), Sorghum bicolor (Sb), Hordeum vulgare (Hv), Brachypodium distachyon (Bd), and Oryza sativa (Os). A total of 105 GAox genes were identified in these six crop genomes, belonging to the C19-GA2ox, C20-GA2ox, GA3ox, and GA20ox subfamilies. Based on orthogroup (OG) analysis, GAox genes were divided into nine OGs and the number of GAox genes in each of the OGs was similar among all tested crops, which indicated that GAox genes may have completed their family differentiations before the species differentiations of the tested species. The motif composition of GAox proteins showed that motifs 1, 2, 4, and 5, forming the 2OG-FeII_Oxy domain, were conserved in all identified GAox protein sequences, while motifs 11, 14, and 15 existed specifically in the GA20ox, C19-GA2ox, and C20-GA2ox protein sequences. Subsequently, the results of gene duplication events suggested that GAox genes mainly expanded in the form of WGD/SD and underwent purification selection and that maize had more GAox genes than other species due to its recent duplication events. The cis-acting elements analysis indicated that GAox genes may respond to growth and development, stress, hormones, and light signals. Moreover, the expression profiles of rice and maize showed that GAox genes were predominantly expressed in the panicles of the above two plants and the expression of several GAox genes was significantly induced by salt or cold stresses. In conclusion, our results provided further insight into GAox genes' evolutionary differences among six representative Gramineae and highlighted GAox genes that may play a role in abiotic stress.

Dataset Information

Functional Analysis of the Gibberellin 2-oxidase Gene Family in Peach.

Publications

Functional Analysis of the <i>Gibberellin 2-oxidase</i> Gene Family in Peach.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets