Ontology highlight
ABSTRACT:
SUBMITTER: Nakase I
PROVIDER: S-EPMC7931216 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Nakase Ikuhiko I Ueno Natsumi N Matsuzawa Mie M Noguchi Kosuke K Hirano Mami M Omura Mika M Takenaka Tomoya T Sugiyama Ayaka A Bailey Kobayashi Nahoko N Hashimoto Takuya T Takatani-Nakase Tomoka T Yuba Eiji E Fujii Ikuo I Futaki Shiroh S Yoshida Tetsuhiko T
FEBS open bio 20210218 3
Exosomes (extracellular vesicles/EVs) participate in cell-cell communication and contain bioactive molecules, such as microRNAs. However, the detailed characteristics of secreted EVs produced by cells grown under low pH conditions are still unknown. Here, we report that low pH in the cell culture medium significantly affected the secretion of EVs with increased protein content and zeta potential. The intracellular expression level and location of stably expressed GFP-fused CD63 (an EV tetraspani ...[more]