Ontology highlight
ABSTRACT:
SUBMITTER: Strittmatter LM
PROVIDER: S-EPMC7935899 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Strittmatter Lisa M LM Capitanchik Charlotte C Newman Andrew J AJ Hallegger Martina M Norman Christine M CM Fica Sebastian M SM Oubridge Chris C Luscombe Nicholas M NM Ule Jernej J Nagai Kiyoshi K
Nature communications 20210305 1
RNA helicases remodel the spliceosome to enable pre-mRNA splicing, but their binding and mechanism of action remain poorly understood. To define helicase-RNA contacts in specific spliceosomal states, we develop purified spliceosome iCLIP (psiCLIP), which reveals dynamic helicase-RNA contacts during splicing catalysis. The helicase Prp16 binds along the entire available single-stranded RNA region between the branchpoint and 3'-splice site, while Prp22 binds diffusely downstream of the branchpoint ...[more]