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Protocol for high-throughput compound screening using flow cytometry in THP-1 cells.

ABSTRACT: Flow cytometry is a valuable method for analyzing protein expressions at the single cell level but can be difficult to apply to large numbers of samples. This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a human monocytic leukemia cell line. We describe a methodology for identifying compounds that regulate PD-L1 surface expression in IFN-γ-stimulated cells, which has been successfully used to screen a collection of ∼200,000 compounds. For complete details on the use and execution of this protocol, please refer to Zavareh et al. (2020).

SUBMITTER: Spangenberg SH

PROVIDER: S-EPMC7985391 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Protocol for high-throughput compound screening using flow cytometry in THP-1 cells.

Spangenberg Stephan H SH Zavareh Reza Beheshti RB Lairson Luke L LL

STAR protocols 20210319 2

Flow cytometry is a valuable method for analyzing protein expressions at the single cell level but can be difficult to apply to large numbers of samples. This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a human monocytic leukemia cell line. We describe a methodology for identifying compounds that regulate PD-L1 surface expression in IFN-γ-stimulated cells, which has been successfully used to screen a collection o ...[more]

PMID: 33778785

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Protocol for high-throughput compound screening using flow cytometry in THP-1 cells.

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Protocol for high-throughput compound screening using flow cytometry in THP-1 cells.

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