The Alphaviral Capsid Protein Inhibits IRAK1-Dependent TLR Signaling.

ABSTRACT: Alphaviruses are arthropod-borne RNA viruses which can cause either mild to severe febrile arthritis which may persist for months, or encephalitis which can lead to death or lifelong cognitive impairments. The non-assembly molecular role(s), functions, and protein-protein interactions of the alphavirus capsid proteins have been largely overlooked. Here we detail the use of a BioID2 biotin ligase system to identify the protein-protein interactions of the Sindbis virus capsid protein. These efforts led to the discovery of a series of novel host-pathogen interactions, including the identification of an interaction between the alphaviral capsid protein and the host IRAK1 protein. Importantly, this capsid-IRAK1 interaction is conserved across multiple alphavirus species, including arthritogenic

SUBMITTER: Landers VD 

PROVIDER: S-EPMC7997285 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature