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The histone modification H3K4me3 is altered at the ANK1 locus in Alzheimer's disease brain.

ABSTRACT: Several epigenome-wide association studies of DNA methylation have highlighted altered DNA methylation in the ANK1 gene in Alzheimer's disease (AD) brain samples. However, no study has specifically examined ANK1 histone modifications in the disease. We use chromatin immunoprecipitation-qPCR to quantify tri-methylation at histone 3 lysine 4 (H3K4me3) and 27 (H3K27me3) in the ANK1 gene in entorhinal cortex from donors with high (n = 59) or low (n = 29) Alzheimer's disease pathology. We demonstrate decreased levels of H3K4me3, a marker of active gene transcription, with no change in H3K27me3, a marker of inactive genes. H3K4me3 is negatively correlated with DNA methylation in specific regions of the ANK1 gene. Our study suggests that the ANK1 gene shows altered epigenetic marks indicative of reduced gene activation in Alzheimer's disease.

SUBMITTER: Smith AR

PROVIDER: S-EPMC8015672 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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The histone modification H3K4me3 is altered at the ANK1 locus in Alzheimer's disease brain.

Smith Adam R AR Smith Rebecca G RG Macdonald Ruby R Marzi Sarah J SJ Burrage Joe J Troakes Claire C Al-Sarraj Safa S Mill Jonathan J Lunnon Katie K

Future science OA 20210209 4

Several epigenome-wide association studies of DNA methylation have highlighted altered DNA methylation in the ANK1 gene in Alzheimer's disease (AD) brain samples. However, no study has specifically examined ANK1 histone modifications in the disease. We use chromatin immunoprecipitation-qPCR to quantify tri-methylation at histone 3 lysine 4 (H3K4me3) and 27 (H3K27me3) in the ANK1 gene in entorhinal cortex from donors with high (n = 59) or low (n = 29) Alzheimer's disease path ...[more]

PMID: 33815817

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