Ontology highlight
ABSTRACT:
SUBMITTER: Choi N
PROVIDER: S-EPMC8016662 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Choi Namjeong N Liu Yongchao Y Oh Jagyeong J Ha Jiyeon J Ghigna Claudia C Zheng Xuexiu X Shen Haihong H
BMB reports 20210301 3
Bcl-x, a member of the Bcl-2 family, plays a key role in apoptosis. Alternative splicing of Bcl-x pre-mRNA through alternative 5' splice-site selection produces an anti-apoptotic mRNA isoform that includes exon 2b and a pro-apoptotic Bcl-x mRNA isoform that excludes exon 2b. Here we used Bcl-x minigene and identified SRSF2 and SRSF6 as two regulatory factors of 5' splice-site selection of Bcl-x pre-mRNA. We selected binding clusters closer to 5' splice-sites from multiple potential binding sites ...[more]