Wavelength-Selective Activation of Photocaged DNAzymes for Metal Ion Sensing in Live Cells.

ABSTRACT: RNA-cleaving DNAzymes are widely applied as sensors for detecting metal ions in environmental samples owing to their high sensitivity and selectivity, but their use for sensing biological metal ions in live cells is challenging because constitutive sensors fail to report the spatiotemporal heterogeneity of biological processes. Photocaged DNAzymes can be activated by light for sensing purposes that need spatial and temporal resolution. Studying complex biological processes requires logic photocontrol, but unfortunately all the literature-reported photocaged DNAzymes working in live cells cannot be selectively controlled by light irradiation at different wavelengths. In this work, we developed photocaged DNAzymes responsive to UV and visible light using a general synthetic method based on p

SUBMITTER: Xu X 

PROVIDER: S-EPMC8158819 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature