Structural insights into acyl-ACP selective recognition by the Aeromonas hydrophila AHL synthase AhyI.

ABSTRACT:

Background

Aeromonas hydrophila is a gram-negative bacterium and the major causative agent of the fish disease motile aeromonad septicemia (MAS). It uses N-acyl-homoserine lactone (AHL) quorum sensing signals to coordinate biofilm formation, motility, and virulence gene expression. The AHL signaling pathway is therefore considered to be a therapeutic target against pathogenic A. hydrophila infection. In A. hydrophila, AHL autoinducers biosynthesis are specifically catalyzed by an ACP-dependent AHL synthase AhyI using the precursors SAM and acyl-ACP. Our previously reported AhyI was heterologously expressed in E. coli, which showed the production characteristics of medium-long chain AHLs. This contradicted the prevailing understanding that AhyI was only a short-chain C₄/C

SUBMITTER: Jin L 

PROVIDER: S-EPMC8188788 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature