Ontology highlight
ABSTRACT:
SUBMITTER: Reinartz I
PROVIDER: S-EPMC8234384 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Reinartz Ines I Sarter Mona M Otten Julia J Höfig Henning H Pohl Martina M Schug Alexander A Stadler Andreas M AM Fitter Jörg J
Sensors (Basel, Switzerland) 20210616 12
Inspired by the modular architecture of natural signaling proteins, ligand binding proteins are equipped with two fluorescent proteins (FPs) in order to obtain Förster resonance energy transfer (FRET)-based biosensors. Here, we investigated a glucose sensor where the donor and acceptor FPs were attached to a glucose binding protein using a variety of different linker sequences. For three resulting sensor constructs the corresponding glucose induced conformational changes were measured by small a ...[more]