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Effects of long-term norepinephrine treatment on normal immortalized ovarian and fallopian tube cells.

ABSTRACT: Sustained adrenergic stimulation by norepinephrine (NE) contributes to ovarian carcinoma metastasis and impairment of chemotherapy response. Although the effect of sustained NE stimulation in cancer progression is well established, less is known about its role in cancer initiation. To determine the extent to which stress hormones influence ovarian cancer initiation, we conducted a long-term (> 3 months; > 40 population doublings) experiment in which normal immortalized fallopian tube secretory (iFTSEC283) and ovarian surface epithelial (iOSE11) cell lines and their isogenic pairs containing a p53 mutation (iFTSEC283^p53R175H; iOSE11^p53R175H), were continuously exposed to NE (100 nM, 1 μM, 10 μM). Fallopian tube cells displayed a p53-independent increase in proliferation and colony-forming ability in response to NE, while ovarian surface epithelial cells displayed a p53-independent decrease in both assays. Fallopian tube cells with mutant p53 showed a mild loss of chromosomes and TP53 status was also a defining factor in transcriptional response of fallopian tube cells to long-term NE treatment.

SUBMITTER: Dash S

PROVIDER: S-EPMC8275603 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Transcriptomic profile of normal immortalized fallopian tube cells in response to long-term norepinephrine treatment

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Transcriptomic profile of normal immortalized fallopian tube cells in response to long-term norepinephrine treatment

Project description:Transcriptomic profile of normal immortalized fallopian tube cells in response to long-term norepinephrine treatment

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Early transcriptional response of human ovarian and fallopian tube surface epithelial cells to norepinephrine.

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Project description:The purpose of this study is to understand the effects of adrenergic signaling on the transcriptome of cell line models postulated to be the cells of origin of epithelial ovarian cancers using RNA-Seq. Here we explored the effects of the stress-related hormone, norepinephrine, on normal human ovarian and fallopian tube surface epithelial cellss. We investigated the early transcriptional response to norepinephrine in normal immortalized ovarian surface epithelial cells and fallopian tube secretory cells. RNA-Seq data of treated and untreated cells were analyzed to identify genes with differential expression.

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Gene signatures of normal hTERT immortalized ovarian epithelium and fallopian tube epithelium (paired cultures from 2 donor patients)

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