Unknown

Dataset Information

0

Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development.


ABSTRACT: Local actin filament formation is indispensable for development of the dendritic arbor of neurons. We show that, surprisingly, the action of single actin filament-promoting factors was insufficient for powering dendritogenesis. Instead, this required the actin nucleator Cobl and its only evolutionary distant ancestor Cobl-like acting interdependently. This coordination between Cobl-like and Cobl was achieved by physical linkage by syndapins. Syndapin I formed nanodomains at convex plasma membrane areas at the base of protrusive structures and interacted with three motifs in Cobl-like, one of which was Ca2+/calmodulin-regulated. Consistently, syndapin I, Cobl-like's newly identified N terminal calmodulin-binding site and the single Ca2+/calmodulin-responsive syndapin-binding motif all were critical for Cobl-like's functions. In dendritic arbor development, local Ca2+/CaM-controlled actin dynamics thus relies on regulated and physically coordinated interactions of different F-actin formation-promoting factors and only together they have the power to bring about the sophisticated neuronal morphologies required for neuronal network formation in mammals.

SUBMITTER: Izadi M 

PROVIDER: S-EPMC8282341 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8699704 | biostudies-literature
| S-EPMC4559358 | biostudies-literature
| S-EPMC7341751 | biostudies-literature
| S-EPMC6621670 | biostudies-literature
| S-EPMC3160182 | biostudies-literature
| S-EPMC9585016 | biostudies-literature
| S-EPMC535351 | biostudies-literature
| S-EPMC5748978 | biostudies-literature
2018-04-30 | PXD008504 | Pride
2017-10-10 | GSE83938 | GEO