A simple microscopy setup for visualizing cellular responses to DNA damage at particle accelerator facilities.

ABSTRACT: Cellular responses to DNA double-strand breaks (DSBs) not only promote genomic integrity in healthy tissues, but also largely determine the efficacy of many DNA-damaging cancer treatments, including X-ray and particle therapies. A growing body of evidence suggests that activation of the mechanisms that detect, signal and repair DSBs may depend on the complexity of the initiating DNA lesions. Studies focusing on this, as well as on many other radiobiological questions, require reliable methods to induce DSBs of varying complexity, and to visualize the ensuing cellular responses. Accelerated particles of different energies and masses are exceptionally well suited for this task, due to the nature of their physical interactions with the intracellular environment, but visualizing cellular respo

SUBMITTER: Qian H 

PROVIDER: S-EPMC8282881 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature