Unknown

Dataset Information

0

E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity.


ABSTRACT: MicroRNAs (miRNAs) are small non-coding RNAs which post-transcriptionally suppress target mRNAs expression and/or translation to modulate pathophyological processes. Expression and function of miRNAs are fine-tuned by a conserved biogenesis machinery involves two RNase-dependent processing steps of miRNA maturation and the final step of miRNA-induced silencing complex (miRISC)-mediated target silencing. A functional miRISC requires Argonaute 2 (AGO2) as an essential catalytic component which plays central roles in miRISC function. We uncovered a post-translational regulatory mechanism of AGO2 by E-cadherin. Mechanistically, E-cadherin activates ERK to phosphorylate AGO2, along with enhanced protein glycosylation. Consequently, the phosphorylated AGO2 was stabilized and ultimately resulted in induced miRISC activity on gene silencing. This study revealed a novel pathway for miRNA regulation through an E-cadherin-mediated miRISC activation.

SUBMITTER: Li JN 

PROVIDER: S-EPMC8287304 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6859290 | biostudies-literature
| S-EPMC2889069 | biostudies-literature
| S-EPMC4758864 | biostudies-literature
| S-EPMC5508727 | biostudies-literature
| S-EPMC2630690 | biostudies-literature
| S-EPMC3794582 | biostudies-literature
| S-EPMC3711048 | biostudies-literature
| S-EPMC5621872 | biostudies-literature
| S-EPMC8183687 | biostudies-literature
| S-EPMC2292232 | biostudies-literature