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A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds.

ABSTRACT: We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.

SUBMITTER: Strobl F

PROVIDER: S-EPMC8319312 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds.

Strobl Frederic F Stelzer Ernst H K EHK

Scientific reports 20210728 1

We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specif ...[more]

PMID: 34321513

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Author Correction: A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds.

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