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Prognostic role of proliferating CD8⁺ cytotoxic Tcells in human cancers.

ABSTRACT:

Purpose

Expansion of CD8⁺ cytotoxic Tlymphocytes is a prerequisite for anti-cancer immune activity and has gained interest in the era of immune checkpoint therapy.

Methods

To understand the CD8⁺ T cell dynamics in the tumor microenvironment, we used multiplex fluorescence immunohistochemistry to quantitate CD8⁺ proliferation (Ki67 co-expression) in tissue microarrays from 1107 colorectal, 642 renal cell, 1066 breast, 375 ovarian, 451 pancreatic and 347 gastric cancer samples.

Results

The density and the percentage of proliferating (Ki67⁺) CD8⁺ T cells were both highly variable between tumor types as well as between patients with the same tumor type. Elevated density and percentage of proliferating CD8⁺ cytotoxic T cells were significantly associated with favorable tumor parameters such as low tumor stage, negative nodal stage (p ≤ 0.0041 each), prolonged overall survival (p ≤ 0.0028 each) and an inflamed immune phenotype (p = 0.0025) in colorectal cancer and, in contrast, linked to high tumor stage, advanced ISUP/Fuhrman/Thoenes grading (each p ≤ 0.003), shorter overall survival (p ≤ 0.0330 each) and an immune inflamed phenotype (p = 0.0094) in renal cell cancer. In breast, ovarian, pancreatic and gastric cancer the role of (Ki67⁺)CD8⁺ Tcells was not linked to clinicopathological data.

Conclusion

Our data demonstrate a tumor type dependent prognostic impact of proliferating (Ki67⁺)CD8⁺ Tcells and an inverse impact in colorectal and renal cell cancer.

SUBMITTER: Blessin NC

PROVIDER: S-EPMC8338812 | biostudies-literature |

REPOSITORIES: biostudies-literature

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