Hemoglobin quantification in red blood cells via dry mass mapping based on UV absorption.

ABSTRACT: The morphological properties and hemoglobin (Hb) content of red blood cells (RBCs) are essential biomarkers to diagnose or monitor various types of hematological disorders. Label-free mass mapping approaches enable accurate Hb quantification from individual cells, serving as promising alternatives to conventional hematology analyzers. Deep ultraviolet (UV) microscopy is one such technique that allows high-resolution, molecular imaging, and absorption-based mass mapping. To compare UV absorption-based mass mapping at four UV wavelengths and understand variations across wavelengths and any assumptions necessary for accurate Hb quantification. Whole blood smears are imaged with a multispectral UV microscopy system, and the RBCs' dry masses are computed. This approach is compared to quantitati

SUBMITTER: Kaza N 

PROVIDER: S-EPMC8353376 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature