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Single red blood cell analysis reveals elevated hemoglobin in poikilocytes.

ABSTRACT: Abnormally shaped red blood cells (RBCs), called poikilocytes, can cause anemia. At present, the biochemical abnormalities in poikilocytes are not well understood. Normal RBCs and poikilocytes were analyzed using whole-blood and single-cell methods. Poikilocytes were induced in rat blood by intragastrically administering titanium dioxide (TiO2) nanoparticles. Complete blood count and inductively coupled plasma mass spectrometry analyses were performed on whole-blood to measure average RBC morphology, blood hemoglobin (HGB), iron content, and other blood parameters. Follow-up confocal Raman spectroscopy was performed on single RBCs to analyze cell-type-specific HGB content. Two types of poikilocytes, acanthocytes and echinocytes, were observed in TiO2 blood samples, along with normal RBCs. Acanthocytes (diameter 7.7??±??0.5???m) and echinocytes (7.6??±??0.6???m) were microscopically larger (p??

SUBMITTER: Tsui SM

PROVIDER: S-EPMC6976897 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Single red blood cell analysis reveals elevated hemoglobin in poikilocytes.

Tsui Suet Man SM Ahmed Rafay R Amjad Noreen N Ahmed Irfan I Yang Jingwei J Manno Francis A FA Barman Ishan I Shih Wei-Chuan WC Lau Condon C

Journal of biomedical optics 20200101 1

Abnormally shaped red blood cells (RBCs), called poikilocytes, can cause anemia. At present, the biochemical abnormalities in poikilocytes are not well understood. Normal RBCs and poikilocytes were analyzed using whole-blood and single-cell methods. Poikilocytes were induced in rat blood by intragastrically administering titanium dioxide (TiO<sub>2</sub>) nanoparticles. Complete blood count and inductively coupled plasma mass spectrometry analyses were performed on whole-blood to measure average ...[more]

PMID: 31975576

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