Single-Cell Transcriptomic Profiling of MAIT Cells in Patients With COVID-19.

ABSTRACT:

Background

Mucosal-associated invariant T (MAIT) cells are considered to participate of the host immune response against acute severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection; however, single-cell transcriptomic profiling of MAIT cells in patients with COVID-19 remains unexplored.

Methods

We performed single-cell RNA sequencing analyses on peripheral MAIT cells from 13 patients with COVID-19 and 5 healthy donors. The transcriptional profiles of MAIT cells, together with assembled T-cell receptor sequences, were analyzed. Flow cytometry analysis was also performed to investigate the properties of MAIT cells.

Results

We identified that differentially expressed genes (DEGs) of MAIT cells were involved in myeloid leukocyte activation and lymphocyte

SUBMITTER: Shi J 

PROVIDER: S-EPMC8363247 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature