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Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants.

ABSTRACT: Iodine deficiency represents a public health problem worldwide. To increase the amount of iodine in the diet, biofortification strategies of plants have been tried. They rely on the exogenous administration of iodine to increase its absorption and accumulation. However, iodine is not stable in plants and can be volatilized as methyl iodide through the action of specific methyltransferases encoded by the HARMLESS TO OZONE LAYER (HOL) genes. The release of methyl iodide in the atmosphere represents a threat for the environment due to its ozone depletion potential. Rice paddies are among the strongest producers of methyl iodide. Thus, the agronomic approach of iodine biofortification is not appropriate for this crop, leading to further increases of iodine emissions. In this work, we used the genome editing CRISPR/Cas9 technology to knockout the rice HOL genes and investigate their function. OsHOL1 resulted a major player in methyl iodide production, since its knockout abolished the process. Moreover, its overexpression reinforced it. Conversely, knockout of OsHOL2 did not produce effects. Our experiments helped elucidating the function of the rice HOL genes, providing tools to develop new rice varieties with reduced iodine emissions and thus more suitable for biofortification programs without further impacting on the environment.

SUBMITTER: Carlessi M

PROVIDER: S-EPMC8382704 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Background: Rice is understood to enhance methane emissions from paddy fields in IPCC guidelines. However, rice actually has two opposite functions related to methane: i) emission enhancement, such as by providing emission pathways (aerenchyma) and methanogenetic substrates; and ii) emission suppression by providing oxygen pathways, which suppress methanogenesis or enhance methane oxidation. The overall role of rice is thus determined by the balance between its enhancing and suppressing functions. Although previous studies have suggested that rice enhances total methane emissions, we aimed to demonstrate in high-emitting paddy fields that the overall methane emission is decreased by rice plants. Methods: We compared methane emissions with and without rice plants in triple cropping rice paddy fields in the Mekong Delta, Vietnam. The gas samples are collected using chamber method and ware analyzed by gas chromatography. Results: We found that rice, in fact, suppressed overall methane emissions in high-emitting paddies. The emission reductions increased with the growth of rice to the maximum tillering stage, then decreased after the heading stage, and finally recovered. Discussion: Our result indicates that the overall methane emission is larger than that of rice planted area. In addition, although many studies in standard-emitting paddies have found that the contribution of soil organic matter to methanogenesis is small, prior studies in high-emitting paddies suggest that methanogenesis depended mainly on soil organic matter accumulated from past crops. The higher the methane emission level, the lower the contribution of the rice-derived substrate; conversely, the higher the contribution of the rice providing oxygen. Finally, rice plants reduce methane emissions in high-emitting paddies. Conclusion: The present study demonstrates that during the growing season, rice is suppressing methane emissions in high-emitting paddies. This means the significance of using the rice variety which has high suppressing performance in high-emitting paddies.

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Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants.

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