A genome-engineered bioartificial implant for autoregulated anticytokine drug delivery.
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ABSTRACT:
SUBMITTER: Choi YR
PROVIDER: S-EPMC8442875 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
A genome-engineered bioartificial implant for autoregulated anticytokine drug delivery.
Choi Yun-Rak YR Collins Kelsey H KH Springer Luke E LE Pferdehirt Lara L Ross Alison K AK Wu Chia-Lung CL Moutos Franklin T FT Harasymowicz Natalia S NS Brunger Jonathan M JM Pham Christine T N CTN Guilak Farshid F
Science advances 20210901 36
Biologic drug therapies are increasingly used for inflammatory diseases such as rheumatoid arthritis but may cause significant adverse effects when delivered continuously at high doses. We used CRISPR-Cas9 genome editing of iPSCs to create a synthetic gene circuit that senses changing levels of endogenous inflammatory cytokines to trigger a proportional therapeutic response. Cells were engineered into cartilaginous constructs that showed rapid activation and recovery in response to inflammation ...[more]
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