CD34+ derived macrophage and dendritic cells display differential responses to paraquat.

ABSTRACT: Paraquat (PQ) is a redox cycling herbicide known for its acute toxicity in humans. Airway parenchymal cells have been identified as primary sites for PQ accumulation, tissue inflammation and cellular injury. However, the role of immune cells in PQ induced tissue injury is largely unknown. To explore this further, primary cultures of human CD34+ stem cell derived macrophages (MC^cd34) and dendritic cells (DC^cd34) were established and characterised using RNA-Seq profiling. The impact of PQ on DC^cd34 and MC^cd34 cytotoxicity revealed increased effect within DC^cd34 cultures. PQ toxicity mechanisms were examined using sub-cytotoxic concentrations and TempO-seq transcriptomic assays. Comparable increases for several stress response pathway (NF

SUBMITTER: Fransen LFH 

PROVIDER: S-EPMC8444090 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature