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First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe.

ABSTRACT: In the last decade, the cultivated area of maize has increased in Central Europe due to its high yield potential and diverse uses for feed and bio-energy. This has led to more intense maize cultivation, with narrowed crop rotations resulting in the increase in maize leaf diseases. During 2012 and 2013, an inventory of maize leaf spot diseases was carried out in various regions in Central Europe. In addition to the major leaf pathogens, isolates of Phoma-like species were obtained from oval to elliptical spots on leaves or found in lesions produced by other leaf pathogens. A total of 16 representative Phoma-like strains were characterised for their pathogenicity on maize leaves, for their morphological characteristics and with a phylogenetic analysis based on multilocus sequence analysis using part of the actin (ACT), calmodulin (CAL), β-tubulin (TUB), internal transcribed spacer (ITS) region of ribosomal DNA and large subunit ribosomal RNA (LSU) genes. The strains were grouped into four clades, and morphological studies supported this classification for most of them. Strains were compared with six reference Phoma-like species strains from the Westerndijk Fungal Biodiversity Institute collection reported to colonise maize. The pathogenic group of strains from our collection (after completion of Koch's postulates) did not cluster with any of these species, indicating a different and novel Phoma-like species infecting maize leaves. To our knowledge, this is the first study dissecting the Phoma species complex on maize leaves in Central Europe.

SUBMITTER: Ramos Romero L

PROVIDER: S-EPMC8467443 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Information on disease process and pathogenicity mechanisms is important for understanding plant disease. Spring black stem and leaf spot caused by the necrotrophic pathogen Phoma medicaginis var. medicaginis Malbr. & Roum causes large losses to alfalfa. However, till now, little is known about alfalfa-P. medicagnis interactions and the pathogenicity mechanisms of the pathogen. Here, alfalfa inoculated with P. medicaginis was subjected to GC-MS based metabolic profiling. The metabolic response in P. medicaginis-inoculated and mock-inoculated alfalfa leaves was assessed at 2, 4, 6, 8, 12, 16, 20, 24, 26 and 28 days post inoculation. In total, 101 peaks were detected in the control and inoculated groups, from which 70 metabolites were tentatively identified. Using multivariate analysis, 16 significantly regulated compounds, including amino acids, nitrogen-containing compounds and organic acids, polyols, fatty acids, and sugars were tentatively identified (Variable importance values, VIP>1.0 and p <0.05). Among these metabolites, the levels of malate, 5-oxoproline, palmitic acid and stearic acid were increased significantly in P. medicaginis-infected alfalfa leaves compared to the controls. In contrast, the levels ofγ-aminobutyric acid and 2-pyrrolidinone were significantly decreased in infected leaves compared to the controls. Further metabolic pathway analysis of the 16 significantly regulated compounds demonstrated that glycolysis, the tricarboxylic acid cycle, and β-oxidation of fatty acids were significantly induced in the alfalfa leaves at later stages of P. medicaginis infection. The strong induction of tricarboxylic acid cycle pathways at later infection stages caused by the pathogen may induce senescence in the alfalfa leaves, leading to plant death. However, intermediate metabolites of these metabolic pathways, and inositol phosphate, glutathione, the metabolic pathways of some amino acids accumulated rapidly and strongly at early stages of infection, which may enhance the ability of alfalfa to resist necrotrophic P. medicaginis disease. Understanding metabolic pathways is essential for understanding pathogenesis.

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First Characterisation of the Phoma Species Complex on Maize Leaves in Central Europe.

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