Dataset Information

Designing of RNA Molecule Translating for Activitable Melittin as Selective Targeting of Leishmania Infected Cells.

ABSTRACT:

Background

Leishmaniasis is characterized by strong inflammatory responses with high levels of inflammatory cytokines that induce microRNA 21 and matrix metalloproteinases. Melittin has inhibitory effects on proliferation of various cells via induction of apoptosis. Melittin can be integrated in cell membranes and induce apoptosis. Thus, designation of biomolecules for the selective destroy of the infected cells is a treatment option. One approach is the precise engineering of constructs for the selective expression of melittin in the infected cells.

Methods

For this aim we designed a construct composing melittin nucleotide sequence and nucleotide sequence coding for polyanionic peptide function inhibitory element to further guarantee the selective function of melittin in inflamed tissues and infected cells, were included in a construct as melittin inhibitor via matrix metalloproteinase degradable linker.

Results

Reverse complementary sequences were designed so melittin sequences for the selective targeting of Leishmania could be expressed in infected cells using cell microRNA machinery.

Conclusion

Translation machinery in infected cells with increased miR-21 could translate melittin, MMP linker and polyanionic inhibitor through a non-canonical pathway. Then, the MMP linker is degraded and selective killing of Leishmania infected cells would happen.

SUBMITTER: Akhzari S

PROVIDER: S-EPMC8476729 | biostudies-literature | 2021 Jul-Sep

REPOSITORIES: biostudies-literature

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Publications

Designing of RNA Molecule Translating for Activitable Melittin as Selective Targeting of <i>Leishmania</i> Infected Cells.

Akhzari Soheila S Nabian Sedigheh S Shayan Parviz P Fard Ramin Mazaheri Nezhad RMN Soltani Minoo M Taheri Mohammad M

Iranian journal of parasitology 20210701 3

<h4>Background</h4>Leishmaniasis is characterized by strong inflammatory responses with high levels of inflammatory cytokines that induce microRNA 21 and matrix metalloproteinases. Melittin has inhibitory effects on proliferation of various cells via induction of apoptosis. Melittin can be integrated in cell membranes and induce apoptosis. Thus, designation of biomolecules for the selective destroy of the infected cells is a treatment option. One approach is the precise engineering of constructs ...[more]

PMID: 34630590

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